4GYP

Crystal structure of the heterotetrameric complex of GlucD and GlucDRP from E. coli K-12 MG1655 (EFI TARGET EFI-506058)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-15
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	C 1 2 1
Unit cell lengths	155.470, 113.020, 128.590
Unit cell angles	90.00, 105.89, 90.00

Refinement procedure

Resolution	29.965 - 2.100
R-factor	0.1505
Rwork	0.149
R-free	0.18630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ec7
RMSD bond length	0.008
RMSD bond angle	1.029
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8_1066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge		0.037	0.609
Number of reflections	118199	2638	18075
<I/σ(I)>	9.24	27.38	2.44
Completeness [%]	99.5	95.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		282	Protein solution was at 12.5 mg/mL containing 20 mM Tris (pH 7.5), 100 mM NaCl, 10 mM EDTA and 10 mM bME. Mother liqueur contained 0.17 M Ammonium acetate, 0.085 M Na-citrate tribasic, dihydrate (pH 5.6), 25.5% PEG 4,000 and 15% v/v glycerol, vapor diffusion, sitting drop, temperature 282K