4GYD
Nostoc sp Cytochrome c6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.721, 79.803, 80.154 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.480 - 1.800 |
| R-factor | 0.18112 |
| Rwork | 0.179 |
| R-free | 0.21564 |
| Structure solution method | SAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.929 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CRANK (Afro/Crunch2/Bp3/DM/ARP-wARP) |
| Refinement software | REFMAC (5.7.0027) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.480 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 44311 | |
| Completeness [%] | 99.5 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.1M Tris, 2.3M Ammonium sulphate, 100mM Lithium sulphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
