4GY7

Crystallographic structure analysis of urease from Jack bean (Canavalia ensiformis) at 1.49 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 63 2 2
Unit cell lengths	140.664, 140.664, 198.109
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.973 - 1.492
R-factor	0.1509
Rwork	0.151
R-free	0.16470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3la4
RMSD bond length	0.007
RMSD bond angle	1.262
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.490	1.490
Number of reflections	180775
Completeness [%]	97.2	97.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	1.6M ammonium phosphate dibasic, 100mM Tris pH 8.8, 10% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 293K