4GY5

Crystal structure of the tandem tudor domain and plant homeodomain of UHRF1 with Histone H3K9me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-02-24
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97927
Spacegroup name	P 42 21 2
Unit cell lengths	145.940, 145.940, 125.903
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.377 - 2.956
R-factor	0.2565
Rwork	0.255
R-free	0.28760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3db3 3shb
RMSD bond length	0.003
RMSD bond angle	0.701
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.1_353))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.956
Number of reflections	27861
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	20% PEG 3350, 200mM Ammonium Tartrate, 100mM Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K