4GY3
T. vulcanus Phycocyanin crystallized in 2M Urea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-23 |
Detector | ADSC QUANTUM 315r |
Spacegroup name | P 63 |
Unit cell lengths | 153.820, 153.820, 39.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.900 - 2.500 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.26200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3018 |
RMSD bond length | 0.012 |
RMSD bond angle | 18.320 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 44.400 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.099 |
Number of reflections | 18972 |
<I/σ(I)> | 13 |
Completeness [%] | 100.0 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 293 | 0.8M ammonium sulfate, 20mM Tris pH=8 mixed 1:1 with 10mg/ml protein + 2M urea, VAPOR DIFFUSION, HANGING DROP, temperature 293K |