4GXF

Role of the biradical intermediate observed during the turnover of SLAC: A two-domain laccase from Streptomyces coelicolor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.93340
Spacegroup name	P 43 21 2
Unit cell lengths	176.660, 176.660, 176.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.010 - 2.734
R-factor	0.18238
Rwork	0.182
R-free	0.19858
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cg8
RMSD bond length	0.010
RMSD bond angle	1.590
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.013
High resolution limit [Å]	2.734	2.730
Number of reflections	59241
Completeness [%]	80.2	80.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	35-45% polyethylene glycol-550-monomethyl ether, 0.15-0.5M NaCl, 0.1M TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K