4GX9
Crystal structure of a DNA polymerase III alpha-epsilon chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-03-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.017, 56.980, 135.073 |
| Unit cell angles | 90.00, 93.78, 90.00 |
Refinement procedure
| Resolution | 35.280 - 2.150 |
| R-factor | 0.22948 |
| Rwork | 0.226 |
| R-free | 0.29079 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hnh |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.745 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.095 | 0.499 |
| Number of reflections | 67257 | |
| <I/σ(I)> | 10.41 | 1.9 |
| Completeness [%] | 97.2 | |
| Redundancy | 3.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 279 | 0.2M MgCl2, 0.1M Tris, 16-18% PEG3350, 3mM TCEP, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
