4GX8
Crystal structure of a DNA polymerase III alpha-epsilon chimera
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.96858 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.470, 56.630, 138.010 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 23.040 - 1.700 |
| R-factor | 0.21442 |
| Rwork | 0.213 |
| R-free | 0.24556 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hnh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.118 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.240 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.107 | 0.396 |
| Number of reflections | 140494 | |
| <I/σ(I)> | 8.6 | 3.7 |
| Completeness [%] | 98.1 | 97.2 |
| Redundancy | 7 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 279 | 0.2M MgCl2, 0.1M Tris, 16-18% PEG3350, 3mM TCEP, pH 8.3, VAPOR DIFFUSION, HANGING DROP, temperature 279K |
