4GWX

Crystal Structure of product complexes of Porcine Liver Fructose-1,6-bisphosphatase with restrained subunit pair rotation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-10-18
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	53.971, 83.272, 165.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.740 - 2.350
R-factor	0.204
Rwork	0.201
R-free	0.26392
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cnq
RMSD bond length	0.021
RMSD bond angle	1.838
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK (9.7L)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.440	41.440	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.094	0.057	0.313
Number of reflections	15914
<I/σ(I)>	7.8	14.3	3.2
Completeness [%]	99.4	97.3	99.9
Redundancy	3.53	3.44	3.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	7.5	298	PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K