4GWS

Crystal Structure of AMP complexes of Porcine Liver Fructose-1,6-bisphosphatase with Filled Central Cavity

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-10-10
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	60.236, 164.285, 79.136
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.190 - 2.750
R-factor	0.20326
Rwork	0.200
R-free	0.26214
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eyj
RMSD bond length	0.023
RMSD bond angle	1.827
Data reduction software	d*TREK (9.7L)
Data scaling software	d*TREK (9.7L)
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0066)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.070	36.070	2.850
High resolution limit [Å]	2.750	5.920	2.750
Rmerge	0.100	0.045	0.272
Number of reflections	17334
<I/σ(I)>	7.5	14.8	3
Completeness [%]	82.0	73.7	85.5
Redundancy	2.89	2.99	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	HANGING DROP	7.5	298	PEG3350, t-butyl alcohol, glycerol, pH 7.5, HANGING DROP, temperature 298K