4GWQ
Structure of the Mediator Head Module from S. cerevisiae in complex with the carboxy-terminal domain (CTD) of RNA Polymerase II Rpb1 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-26 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 142.449, 142.449, 305.641 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.000 - 4.500 |
| R-factor | 0.289 |
| Rwork | 0.286 |
| R-free | 0.34500 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.560 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 123.365 | 4.700 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Number of reflections | 21971 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 14.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 291.2 | 275mM ammonium citrate tribasic pH 7.0, 10% w/v PEG 3350, 2mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 291.2K |
