4GW4
Crystal structure of 3BNC60 Fab with P61A mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.570, 154.880, 74.240 |
| Unit cell angles | 90.00, 109.75, 90.00 |
Refinement procedure
| Resolution | 39.346 - 2.650 |
| R-factor | 0.2154 |
| Rwork | 0.213 |
| R-free | 0.25560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_966)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.350 | 2.720 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 39093 | |
| Completeness [%] | 97.9 | 96.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.9 | 293 | 16% polyethylene glycol 6,000, 0.1 M citric acid pH 3.9, 0.15 M lithium sulfate monohydrate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
