4GVR
X-ray structure of the Archaeoglobus fulgidus methenyl-tetrahydromethanopterin cyclohydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Collection date | 2011-12-08 |
| Wavelength(s) | 1.0001 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.540, 122.080, 79.390 |
| Unit cell angles | 90.00, 99.02, 90.00 |
Refinement procedure
| Resolution | 48.170 - 1.520 |
| R-factor | 0.16754 |
| Rwork | 0.164 |
| R-free | 0.23124 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qlm |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.890 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 48.170 |
| High resolution limit [Å] | 1.520 |
| Number of reflections | 164493 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 291 | 10% (w/v) PEG 4000, 10% (v/v) Isopropanol, 0.1M Sodium acetate pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
