4GVF
Crystal structure of Salmonella typhimurium family 3 glycoside hydrolase (NagZ) bound to GlcNAc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-19 |
| Detector | RAYONIX MX-300 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.544, 66.233, 94.968 |
| Unit cell angles | 90.00, 99.32, 90.00 |
Refinement procedure
| Resolution | 48.890 - 1.350 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.066 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.890 | 1.420 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.060 | 0.234 |
| Number of reflections | 131665 | |
| <I/σ(I)> | 13.8 | 4.7 |
| Completeness [%] | 99.1 | 93.7 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 296 | 0.1M MES, 25% PEG 1000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
