4GV6
Structures of Lassa and Tacaribe viral nucleoproteins with or without 5 triphosphate dsRNA substrate reveal a unique 3 -5 exoribonuclease mechanism to suppress type I interferon production
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 176 |
| Detector technology | CCD |
| Collection date | 2011-06-06 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 46.510, 46.510, 208.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.160 - 1.980 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.22900 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.001 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.540 | 2.080 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.050 | 0.175 |
| Number of reflections | 16160 | |
| <I/σ(I)> | 8 | 2.2 |
| Completeness [%] | 89.8 | 92.4 |
| Redundancy | 2.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 18.5% PEG MME 5000, 0.1 M sodium citrate, and 2.4% PEG MME350, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
