4GUR
Crystal structure of LSD2-NPAC with H3 in space group P21
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97908 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.243, 89.033, 88.783 |
Unit cell angles | 90.00, 103.30, 90.00 |
Refinement procedure
Resolution | 38.867 - 2.506 |
R-factor | 0.1888 |
Rwork | 0.188 |
R-free | 0.20450 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gu1 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.372 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Number of reflections | 28816 |
Completeness [%] | 88.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 0.02M Citric acid, 0.03M Bis_tris propane, 10% PEG3350, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |