4GU1
Crystal structure of LSD2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97947 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 89.163, 89.216, 342.538 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.562 - 2.939 |
R-factor | 0.1872 |
Rwork | 0.185 |
R-free | 0.22790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2v1d |
RMSD bond length | 0.011 |
RMSD bond angle | 1.342 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 57805 |
Completeness [%] | 91.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.2M Sodium chloride, 0.1M Na/K phosphate, 7% PEG 8000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |