4GTR
FTase in complex with BMS analogue 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 61 |
| Unit cell lengths | 172.275, 172.275, 69.607 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.910 - 2.200 |
| R-factor | 0.18039 |
| Rwork | 0.179 |
| R-free | 0.21229 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eu5 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | XSCALE |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.910 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 60023 | |
| <I/σ(I)> | 18.8 | 4.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 5.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 20% (w/v) PEG 4000, 0.2M MgCl2, 0.1M Tris, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
