4GTL
T. Maritima FDTS (R174K mutant) with FAD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.780, 116.460, 141.190 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.050 - 2.170 |
| R-factor | 0.19198 |
| Rwork | 0.189 |
| R-free | 0.24073 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1o26 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.725 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.230 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rmerge | 0.065 | |
| Number of reflections | 47764 | |
| <I/σ(I)> | 18.1 | 1.9 |
| Completeness [%] | 99.8 | 99.5 |
| Redundancy | 5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 50-60% PEG 200 and 100mM Tris HCL (pH 8.0), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
