4GT7
An engineered disulfide bond reversibly traps the IgE-Fc3-4 in a closed, non-receptor binding conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2004-11-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.007570 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.753, 104.807, 45.899 |
| Unit cell angles | 90.00, 94.51, 90.00 |
Refinement procedure
| Resolution | 29.204 - 2.610 |
| R-factor | 0.1924 |
| Rwork | 0.190 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.194 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.770 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Rmerge | 0.069 | 0.354 |
| Number of reflections | 29836 | |
| <I/σ(I)> | 13.8 | 3.99 |
| Completeness [%] | 97.1 | 93.7 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 298 | 20 mM sodium acetate, pH 4.6, 28.8% (w/v) PEG 4000, 2% (w/v) Anapoe -X-405, VAPOR DIFFUSION, temperature 298K |
