4GT5
Crystal structure of the inactive TrkA kinase domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2011-03-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03321 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 105.033, 105.033, 203.316 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.384 - 2.398 |
| R-factor | 0.2039 |
| Rwork | 0.202 |
| R-free | 0.24790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1luf |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.398 | 2.398 |
| Number of reflections | 17203 | |
| <I/σ(I)> | 52.9 | 5.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 11.1 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294.15 | 1.5 M sodium chloride, 0.1 M MES, pH 6.5, 0.2 M sodium/potassium phosphate, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
