4GS3
Dimeric structure of the N-terminal domain of PriB protein from Thermoanaerobacter tencongensis solved ab initio
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 55.560, 41.180, 38.780 |
| Unit cell angles | 90.00, 94.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.090 |
| R-factor | 0.1292 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.800 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.090 |
| Rmerge | 0.047 |
| Number of reflections | 38376 |
| <I/σ(I)> | 29.3 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.14 M ammonium acetate, 0.07 M acetate buffer pH 4.6, 21% PEG 4K, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
