4GRV
The crystal structure of the neurotensin receptor NTS1 in complex with neurotensin (8-13)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.960, 69.619, 97.550 |
| Unit cell angles | 90.00, 101.75, 90.00 |
Refinement procedure
| Resolution | 14.958 - 2.802 |
| R-factor | 0.2284 |
| Rwork | 0.225 |
| R-free | 0.28180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ny9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.014 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER ((PHENIX)) |
| Refinement software | PHENIX ((phenix.refine: dev_1042)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 14956 | |
| <I/σ(I)> | 1.3 | |
| Completeness [%] | 86.4 | |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Lipid cubic phase (LCP) | 7.4 | 298 | 80 mM HEPES pH 7.0, 2 mM TCEP, 43 mM NaK tartrate, 20.8% PEG400, Lipid cubic phase (LCP), temperature 298K |
