4GRP

Crystallographic and biological characterization of N- and C- terminus mutants of human MIF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-10
Detector	PSI PILATUS 6M
Spacegroup name	P 2 21 21
Unit cell lengths	50.050, 76.250, 82.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.840 - 1.270
R-factor	0.138
Rwork	0.137
R-free	0.16900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	MIF
RMSD bond length	0.014
RMSD bond angle	1.484
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.730	1.700
High resolution limit [Å]	1.270	1.340
Rmerge	0.090
Number of reflections	81548
<I/σ(I)>	10.8	1.8
Completeness [%]	97.6	48.8
Redundancy	8.2	0.46

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	293	1.6M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K