4GR7

The human W42R Gamma D-Crystallin Mutant Structure at 1.7A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	93
Detector technology	CCD
Collection date	2012-05-04
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54
Spacegroup name	P 32 2 1
Unit cell lengths	68.607, 68.607, 133.382
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.500 - 1.700
R-factor	0.20224
Rwork	0.200
R-free	0.24458
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk0
RMSD bond length	0.008
RMSD bond angle	1.081
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.500	1.740
High resolution limit [Å]	1.700	1.700
Rmerge		0.574
Completeness [%]	92.1	84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.18 M Li2SO4, 0.1 M Tris-HCl, 22% (w/v) PEG 4000, 15% (v/v) glycerol , pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K