4GQT
N-terminal domain of C. elegans Hsp90
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-05 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.652, 86.658, 128.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.300 - 2.150 |
| R-factor | 0.1973 |
| Rwork | 0.194 |
| R-free | 0.25190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jjc |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.873 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.300 | 50.000 | 2.190 |
| High resolution limit [Å] | 2.150 | 5.830 | 2.150 |
| Rmerge | 0.104 | 0.058 | 0.835 |
| Number of reflections | 24707 | ||
| <I/σ(I)> | 7.2 | 2.18 | |
| Completeness [%] | 98.4 | 97 | 97.1 |
| Redundancy | 7 | 6.4 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.08 M zinc acetate, 19% PEG-3350, 0.01 M ADP, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
