4GPY

Crystal structure of the bacterial ribosomal decoding site in complex with 6'-hydroxysisomicin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-16
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	33.742, 84.715, 47.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
Rwork	0.201
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.969
Data reduction software	CrystalClear
Data scaling software	CrystalClear
Phasing software	PHENIX
Refinement software	CNS (1.3)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.800
High resolution limit [Å]	2.800
Number of reflections	3348

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	Na Cacodylate, Spermine, NaCl, MPD , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K