4GPS
Crystal Structure of K. lactis Dxo1 (YDR370C)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979, 0.9793, 0.9724 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 82.820, 82.820, 259.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.930 - 2.400 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 20835 |
| Completeness [%] | 95.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2M Sodium citrate tribasic, 23.5% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
