4GP4

Structure of Recombinant Cytochrome ba3 Oxidase mutant Y133F from Thermus thermophilus

Replaces:  4F05

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	144.140, 98.580, 94.800
Unit cell angles	90.00, 127.89, 90.00

Refinement procedure

Resolution	74.820 - 2.800
R-factor	0.18175
Rwork	0.179
R-free	0.22666
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.131
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	74.820
High resolution limit [Å]	2.800
Number of reflections	25916
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	293	50mM Sodium Cacodylate, 1.6M NaCl, 40% PEG400, pH 6.5, Lipidic Cubic Phase, temperature 293K