4GOW

Crystal Structure of Ca2+/CaM:Kv7.4 (KCNQ4) B helix complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Detector technology	CCD
Wavelength(s)	1.006
Spacegroup name	P 65 2 2
Unit cell lengths	104.075, 104.075, 113.729
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.093 - 2.600
R-factor	0.2379
Rwork	0.236
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.790
Data reduction software	MOSFLM (3.3.9)
Data scaling software	SCALA (3.3.9)
Phasing software	PHASER
Refinement software	PHENIX (1.7.2_869)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	90.131	113.730	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.041	0.965
Number of reflections	11737
<I/σ(I)>	18.9	14.3	0.8
Completeness [%]	100.0	99.6	100
Redundancy	16.7	14.2	12.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	298	1.6M ammonium sulfate, 0.2M sodium citrate (pH 6.0), 0.1M sodium tartrate, 4% isopropanol, VAPOR DIFFUSION, HANGING DROP, temperature 298K