4GOQ

Crystal structure of a DUF1491 family protein (CC_1065) from Caulobacter crescentus CB15 at 1.87 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97849,0.91837,0.97898
Spacegroup name	P 31 2 1
Unit cell lengths	59.730, 59.730, 323.010
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.262 - 1.870
R-factor	0.1818
Rwork	0.180
R-free	0.21060
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	0.890
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.262	49.260	1.940
High resolution limit [Å]	1.870	4.020	1.870
Rmerge	0.075	0.032	0.902
Number of reflections	56900	6150	5785
<I/σ(I)>	16.3	45.8	2.27
Completeness [%]	99.7	99.2	99.5
Redundancy	6.45		6.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2M magnesium chloride, 2.5M sodium chloride, 0.1M TRIS pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K