4GOC

Crystal structure of the Get5 ubiquitin-like domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-26
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	49.360, 63.620, 71.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.069 - 2.400
R-factor	0.1809
Rwork	0.174
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.103
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1066))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.070	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.133	0.613
Number of reflections	9092
<I/σ(I)>	6.6	1.8
Completeness [%]	98.2	98.7
Redundancy	3.3	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.4 M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K