4GO8
Crystal Structure of the TREHALULOSE SYNTHASE MUTB, MUTANT A258V, in complex with TRIS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-11 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97559 |
Spacegroup name | P 1 |
Unit cell lengths | 63.510, 71.310, 81.530 |
Unit cell angles | 68.04, 72.72, 70.10 |
Refinement procedure
Resolution | 14.820 - 2.150 |
R-factor | 0.194 |
Rwork | 0.190 |
R-free | 0.24950 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1zjb |
RMSD bond length | 0.003 |
RMSD bond angle | 0.665 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.820 | 2.300 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.314 | |
Number of reflections | 62687 | |
<I/σ(I)> | 7.6 | 3.46 |
Completeness [%] | 94.0 | 93.3 |
Redundancy | 1.9 | 1.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 0.1M Tris-HCl, 12%(w/v) PEG20000, 0.01M L-Cysteine, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 292K |