4GO1
Crystal Structure of full length transcription repressor LsrR from E. coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-22 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9999 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 79.702, 113.866, 185.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.21969 |
| Rwork | 0.216 |
| R-free | 0.26759 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w48 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.319 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.092 | 0.283 |
| Number of reflections | 17219 | |
| <I/σ(I)> | 21.2 | 5.2 |
| Completeness [%] | 99.5 | 95.1 |
| Redundancy | 7.3 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1M Hepes, 6%(w/v) PEG8000, 8%(v/v) ethylene glycol, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
