4GO0
Crystal structure of the c707s mutant of c-terminal domain of 10'formyltetrahydrofolate dehydrogenase in complex with NADPH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 258.420, 194.131, 96.970 |
| Unit cell angles | 90.00, 108.82, 90.00 |
Refinement procedure
| Resolution | 52.500 - 3.380 |
| R-factor | 0.19804 |
| Rwork | 0.195 |
| R-free | 0.25169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o2p |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.920 |
| Data reduction software | HKL-2000 |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.500 | 3.520 |
| High resolution limit [Å] | 3.380 | 3.400 |
| Rmerge | 0.199 | 0.553 |
| Number of reflections | 62155 | |
| <I/σ(I)> | 6.4 | 2.1 |
| Completeness [%] | 98.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 1.5 M Ammonium Sulfate, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
