4GNT
Complex of ChREBP and 14-3-3beta
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Collection date | 2012-03-01 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 115.864, 115.864, 59.470 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.740 - 2.410 |
| R-factor | 0.1994 |
| Rwork | 0.197 |
| R-free | 0.23866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.796 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 33.700 |
| High resolution limit [Å] | 2.410 |
| Number of reflections | 28628 |
| Completeness [%] | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100mM sodium citrate, 200mM sodium/potassium tartrate, 2.2M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
