4GNI

Structure of the Ssz1 ATPase bound to ATP and Magnesium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-04
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.87
Spacegroup name	P 1 21 1
Unit cell lengths	80.630, 55.380, 98.790
Unit cell angles	90.00, 106.53, 90.00

Refinement procedure

Resolution	36.066 - 1.796
R-factor	0.2021
Rwork	0.201
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.107
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.070	1.890
High resolution limit [Å]	1.796	1.796
Rmerge	0.104
Number of reflections	78273
<I/σ(I)>	9
Completeness [%]	100.0	100
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	0.1 M HEPES, pH 7.5; 20 % PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K