4GN1
Crystal Structure of the RA and PH domains of Lamellipodin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.340, 93.465, 80.720 |
| Unit cell angles | 90.00, 104.91, 90.00 |
Refinement procedure
| Resolution | 46.730 - 2.400 |
| R-factor | 0.256 |
| Rwork | 0.253 |
| R-free | 0.30300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tca |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.409 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.730 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 41656 | |
| Completeness [%] | 99.2 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 200 mM sodium malonate pH 5.0, 20% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
