4GMV
Crystal Structure of the coiled-coil, RA and PH domains of Lamellipodin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-19 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.617, 96.972, 76.824 |
Unit cell angles | 90.00, 126.99, 90.00 |
Refinement procedure
Resolution | 37.960 - 2.403 |
R-factor | 0.2421 |
Rwork | 0.240 |
R-free | 0.28254 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3tca |
RMSD bond length | 0.007 |
RMSD bond angle | 1.151 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.000 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 24426 | |
Completeness [%] | 95.4 | 79.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 50 mM sodium formate, 20% (w/v) polyethylene glycol 3350, 30% (v/v) ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |