4GM9
Crystal structure of human WD repeat domain 5 with compound MM-401
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-02-25 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.904, 47.242, 68.896 |
| Unit cell angles | 88.50, 89.50, 74.53 |
Refinement procedure
| Resolution | 37.822 - 2.100 |
| R-factor | 0.1613 |
| Rwork | 0.159 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h14 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Number of reflections | 32452 | ||
| <I/σ(I)> | 14.2 | 3.8 | |
| Completeness [%] | 97.8 | 97.7 | 94.9 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG4000, 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.00K |
