4GLU
Crystal structure of the mirror image form of VEGF-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 231.395, 43.986, 73.514 |
| Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
| Resolution | 32.499 - 1.900 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qtk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.146 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1128)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.071 | 0.625 |
| Number of reflections | 57494 | |
| <I/σ(I)> | 13.16 | 2.3 |
| Completeness [%] | 98.5 | 99.4 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 292 | 0.2 M NH4OAc, 0.1 M BIS-TRIS (pH 5.5), 45% (v/v) 2-methyl-2,4-pentanediol, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
