4GLT
Crystal structure of glutathione s-transferase MFLA_2116 (target EFI-507160) from methylobacillus flagellatus kt with gsh bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.815, 97.024, 81.868 |
| Unit cell angles | 90.00, 105.38, 90.00 |
Refinement procedure
| Resolution | 46.460 - 2.200 |
| R-factor | 0.21139 |
| Rwork | 0.209 |
| R-free | 0.28251 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tou |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.060 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0025) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.860 | |
| Number of reflections | 42092 | |
| <I/σ(I)> | 8.3 | 1.2 |
| Completeness [%] | 99.3 | 95 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.2M POTASSIUM ACETATE, PH 7.5, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 294K |
