4GLM
Crystal structure of the SH3 Domain of DNMBP protein [Homo sapiens]
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 58.279, 136.203, 33.623 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.820 - 1.900 |
| R-factor | 0.2302 |
| Rwork | 0.229 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2drk |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
| Rmerge | 0.080 | 0.031 | 0.757 |
| Number of reflections | 21923 | ||
| <I/σ(I)> | 9 | ||
| Completeness [%] | 99.9 | 99.4 | 100 |
| Redundancy | 7.5 | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 291 | 5% PEG 8000, 0.2 M NaCl, and 0.1 M Tris 8.5, vapor diffusion hanging drop, temperature 291K |
