4GLI
Crystal Structure of Human SMN YG-Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-02-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 87.745, 65.914, 84.908 |
| Unit cell angles | 90.00, 110.48, 90.00 |
Refinement procedure
| Resolution | 26.734 - 1.903 |
| R-factor | 0.20533 |
| Rwork | 0.203 |
| R-free | 0.24709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omp |
| RMSD bond length | 0.024 |
| RMSD bond angle | 1.982 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.734 | |
| High resolution limit [Å] | 1.903 | 1.903 |
| Rmerge | 0.126 | 0.560 |
| Number of reflections | 35714 | |
| <I/σ(I)> | 19.7 | 2.4 |
| Completeness [%] | 99.4 | 98.8 |
| Redundancy | 5.3 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294.15 | 0.1 M Tris-HCl, pH 8.0, 14% w/v PEG3350, 10 mM calcium chloride, 0.1 M potassium chloride, 0.1 M ammonium sulfate, 18% ethylene glycol, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
