4GKQ

Structure of the neck and C-terminal motor homology domain of ViK1 from Candida glabrata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.033
Spacegroup name	H 3
Unit cell lengths	197.000, 197.000, 50.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.800 - 2.990
R-factor	0.2182
Rwork	0.214
R-free	0.25940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.160
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		3.070
High resolution limit [Å]	2.990	13.370	2.990
Rmerge	0.088	0.072	0.928
Number of reflections	14501	72	1076
<I/σ(I)>	13.14	17.99	2.13
Completeness [%]	98.8	43.1	100
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	0.1M Tris, 12% PEG8000, 0.05M MgCl2, 0.15M NaCl, 5% Ethylene glycol, 1mM TCEP, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K