4GKP

Structure of the truncated neck and C-terminal motor homology domain of ViK1 from Candida glabrata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-10-05
Detector	ADSC QUANTUM 270
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	66.742, 77.722, 107.737
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 2.420
R-factor	0.212
Rwork	0.209
R-free	0.25930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o0a
RMSD bond length	0.009
RMSD bond angle	1.255
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.500
High resolution limit [Å]	2.420	10.000	2.420
Rmerge	0.035	0.025	0.193
Number of reflections	21752	272	1880
<I/σ(I)>	37.65	69.06	8.17
Completeness [%]	98.8	74.1	92.7
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1M HEPES, 13% PEG-6000, 5% isopropanol, 75mM NaAcetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K