4GKC
Crystal structure of Q108K:K40L:T51V:T53C:R58W:T29L:Y19W:Q4A mutant of cellular retinol binding protein II complex with all-trans-retinal at 1.33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Detector | MAR300 |
| Wavelength(s) | 1.1272 |
| Spacegroup name | P 1 |
| Unit cell lengths | 30.970, 36.002, 64.016 |
| Unit cell angles | 86.10, 86.46, 65.32 |
Refinement procedure
| Resolution | 28.120 - 1.300 |
| R-factor | 0.18897 |
| Rwork | 0.187 |
| R-free | 0.22493 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2rcq |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.491 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 28.122 |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 50433 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 298 | 40% PEG 4000, 0.1M CH3COONa.3H2O, 0.1 M CH3COONH4, EVAPORATION, temperature 298K, pH 4.6 |
