4GK8

Crystal structure of histidinol phosphate phosphatase (HISK) from Lactococcus lactis subsp. lactis Il1403 complexed with ZN and L-histidinol arsenate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 2
Unit cell lengths	85.860, 86.622, 45.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.311 - 1.933
R-factor	0.1633
Rwork	0.162
R-free	0.19550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gc3
RMSD bond length	0.007
RMSD bond angle	1.082
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.311
High resolution limit [Å]	1.933
Number of reflections	25741
Completeness [%]	99.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	25% SODIUM CHLORIDE, 0.1M IMIDAZOLE, 0.5 mM Zinc Chloride, 100 mM sodium arsenate, 50mM L-histidinol.HCl PH 8.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293.0K