4GK5
Crystal structure of human Rev3-Rev7-Rev1-Polkappa complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.998, 72.762, 104.653 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.210 |
| R-factor | 0.23408 |
| Rwork | 0.232 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gk0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.615 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.310 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.087 | 0.431 |
| Number of reflections | 14623 | |
| <I/σ(I)> | 21.5 | 5.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | soaking the Rev3-Rev7-Rev1 crystals with the RIR peptide of Polkappa | 5.8 | 289 | 0.1M sodium citrate, 1.95M sodium formate, 20mM DTT , pH 5.8, soaking the Rev3-Rev7-Rev1 crystals with the RIR peptide of Polkappa, temperature 289K |
