4GK0
Crystal structure of human Rev3-Rev7-Rev1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 121.089, 71.887, 106.305 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.22094 |
| Rwork | 0.218 |
| R-free | 0.27338 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3abd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.554 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.063 | 0.383 |
| Number of reflections | 23773 | |
| <I/σ(I)> | 22.3 | 3.3 |
| Completeness [%] | 95.9 | 97.5 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 289 | 0.1M sodium citrate, 1.95M sodium formate, 20mM DTT , pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
